In Vivo Rat Facts
  • 37 compounds
  • Complete rat data set includes:

  Portal Vein infusion (30 min)/ systemic sampling
  IV bolus (tail vein)/ systemic sampling
  IV bolus (tail vein)/ portal vein sampling
  PO gavage/ systemic sampling
  PO gavage/ portal vein sampling

  • All samples run in one laboratory
  • All compounds have corresponding in vitro data
  • All compounds have corresponding human data 
  • Raw data profiles available
  • Bioanalysis protocol available
  • Complete study protocol available

In Vivo Rat Dataset

 

Description

The purpose of this study was to evaluate the plasma concentration profiles of test compounds in male Sprague-Dawley rats following single dose administration by the oral (p.o.), intravenous (i.v.) or portal vein (p.v.) routes, with sampling from the systemic or portal circulation.  Data derived from these studies may be used for building a training set or validation data for Rat ADME/PBPK Models. 

 

Method

Compounds were administered as a solution/suspension by oral gavage or as a solution (maximum 10% ethanol) by intravenous or portal vein routes.  Rats were randomly divided into treatment groups of 4 male rats per group.  Each animal received a single dose of a test compound administered at 30 mg/kg body weight by oral gavage or 5 mg/kg as a bolus intravenous or portal vein infusion dose.   Systemic blood samples were collected directly from the jugular vein using jugular vein-cannulated rats, following p.o., i.v. and p.v. dose administration.   

Portal vein blood samples were collected from separate portal vein-cannulated rats, following p.o. and i.v dose administration.  Systemic and portal blood samples were collected from each rat according to the scheduled postdosing:  p.o. group:  0 (predose), 15, 30, 60 minutes and 1.5 (systemic sampling only), 2, 4, 6, 8 and 24 hours; i.v. and p.v. groups: 0 (predose), 1, 5, 15, 30, 60 minutes and 2, 4, 6, 8 and 24 hours.

Plasma fractions were analyzed for parent drug concentrations. 

This dataset also includes 30 compounds assay results for intestinal permeability (duodenum, jejunum, ileum and colon) in rats.

To review a sample  PK report for one compound (gancyclovir) click here